HNF1B Chemi-Luminescent ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

HNF1 homeobox B

Gene Aliases

Hepatocyte nuclear factor 1-beta; HNF-1B; HNF-1-beta; LF-B3; Tcf2; Transcription factor 2; Transcription factor 2, hepatic; variant hepatic nuclear factor 1; VHNF1.

Gene ID

25640

Accession Number

NP_037235

Reactivity

Rat|Rattus norvegicus

Target

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Beta (HNF1b) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates and other biological fluids

Detection Range

13.7-10,000pg/mL

Sensitivity

0.156 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Hnf1b Microplate 96 Wells (12 x 8 Well strips) Hnf1b Lyophilized Standard 2 100X Hnf1b Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Hnf1b Microplate 96 Wells (12 x 8 Well strips) Hnf1b Microplate 96 Wells (12 x 8 Well strips) Hnf1b Lyophilized Standard 2 Hnf1b Lyophilized Standard 2 100X Hnf1b Biotinylated Detector Anitbody 1 x 120 uL 100X Hnf1b Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL