What is the catalog number for HIF1A Chemi-Luminescent ELISA Kit (Pig)?

The catalog number for HIF1A Chemi-Luminescent ELISA Kit (Pig) is OKCD03559-96W.

What sizes are available for HIF1A Chemi-Luminescent ELISA Kit (Pig)?

HIF1A Chemi-Luminescent ELISA Kit (Pig) is available in the following size(s): 96 Well.

Is HIF1A Chemi-Luminescent ELISA Kit (Pig) in stock?

HIF1A Chemi-Luminescent ELISA Kit (Pig) is currently made to order / not held in immediate stock. Contact us for lead time.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

HIF1A Chemi-Luminescent ELISA Kit (Pig)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Hypoxia inducible factor 1 subunit alpha

Gene Aliases

Hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1-alpha.

Gene ID

396696

Accession Number

NP_001116596

Reactivity

Porcine|Sus scrofa

Target

Hypoxia-inducible factor 1alpha

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hypoxia Inducible Factor 1 Alpha (HIF1a) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 EDTA Plasma (n=5) 83-95 90 Heparin Plasma (n=5) 78-98 83 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 Serum (n=5) 97-105 101 EDTA Plasma (n=5) 83-95 90 EDTA Plasma (n=5) 83-95 90 Heparin Plasma (n=5) 78-98 83 Heparin Plasma (n=5) 78-98 83

Sensitivity

15.6 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Hif1a Microplate 96 Wells (12 x 8 Well strips) Hif1a Lyophilized Standard 2 100X Hif1a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Hif1a Microplate 96 Wells (12 x 8 Well strips) Hif1a Microplate 96 Wells (12 x 8 Well strips) Hif1a Lyophilized Standard 2 Hif1a Lyophilized Standard 2 100X Hif1a Biotinylated Detector Anitbody 1 x 120 uL 100X Hif1a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL