What is the catalog number for FOLR1 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for FOLR1 Chemi-Luminescent ELISA Kit (Human) is OKCD03498-96W.

What sizes are available for FOLR1 Chemi-Luminescent ELISA Kit (Human)?

FOLR1 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is FOLR1 Chemi-Luminescent ELISA Kit (Human) in stock?

FOLR1 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

FOLR1 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Folate receptor alpha

Gene Aliases

Adult folate-binding protein; FBP; folate binding protein; Folate receptor 1; folate receptor 1 (adult) ; folate receptor alpha; folate receptor, adult; FOLR; FRalpha; FR-alpha; KB cells FBP; NCFTD; ovarian tumor-associated antigen MOv18.

Gene ID

2348

Accession Number

NP_000793

Reactivity

Homo sapiens|Human

Target

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Folate Receptor 1, Adult (FOLR1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Folate Receptor 1, Adult (FOLR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Folate Receptor 1, Adult (FOLR1) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

1.37-1,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 94-105 101 EDTA Plasma (n=5) 96-103 101 Heparin Plasma (n=5) 89-98 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94-105 101 Serum (n=5) 94-105 101 EDTA Plasma (n=5) 96-103 101 EDTA Plasma (n=5) 96-103 101 Heparin Plasma (n=5) 89-98 93 Heparin Plasma (n=5) 89-98 93

Sensitivity

1.37 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount FOLR1 Microplate 96 Wells (12 x 8 Well strips) FOLR1 Lyophilized Standard 2 100X FOLR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount FOLR1 Microplate 96 Wells (12 x 8 Well strips) FOLR1 Microplate 96 Wells (12 x 8 Well strips) FOLR1 Lyophilized Standard 2 FOLR1 Lyophilized Standard 2 100X FOLR1 Biotinylated Detector Anitbody 1 x 120 uL 100X FOLR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL