What is the catalog number for FGF11 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for FGF11 Chemi-Luminescent ELISA Kit (Human) is OKCD03464-96W.

What sizes are available for FGF11 Chemi-Luminescent ELISA Kit (Human)?

FGF11 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is FGF11 Chemi-Luminescent ELISA Kit (Human) in stock?

FGF11 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

FGF11 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Fibroblast Growth Factor 11 (FGF11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Fibroblast Growth Factor 11 (FGF11) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 11

Gene Aliases

FGF-11; FHF3; FHF-3; fibroblast growth factor 11; fibroblast growth factor homologous factor 3.

Gene ID

2256

Accession Number

NP_001290389

Reactivity

Homo sapiens|Human

Target

Probably involved in nervous system development and function.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 11 (FGF11) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 11 (FGF11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 11 (FGF11) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 96-104 101 EDTA Plasma (n=5) 80-94 88 Heparin Plasma (n=5) 96-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-104 101 Serum (n=5) 96-104 101 EDTA Plasma (n=5) 80-94 88 EDTA Plasma (n=5) 80-94 88 Heparin Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 96-105 101

Sensitivity

15.63 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount FGF11 Microplate 96 Wells (12 x 8 Well strips) FGF11 Lyophilized Standard 2 100X FGF11 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount FGF11 Microplate 96 Wells (12 x 8 Well strips) FGF11 Microplate 96 Wells (12 x 8 Well strips) FGF11 Lyophilized Standard 2 FGF11 Lyophilized Standard 2 100X FGF11 Biotinylated Detector Anitbody 1 x 120 uL 100X FGF11 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL