EPGN Chemi-Luminescent ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Epigen (EPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Epigen (EPG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Epithelial mitogen
Gene Aliases
ALGV3072; EPG; epigen; Epithelial mitogen; epithelial mitogen homolog; PRO9904.
Gene ID
255324
Accession Number
NP_001257918
Reactivity
Homo sapiens|Human
Target
Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Type
ELISA Kit
Applications
Chemiluminescent immunoassay
Detection Method
Chemiluminescent
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Epigen (EPG) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epigen (EPG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epigen (EPG) level in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids
Detection Range
27.4-20,000pg/mL
Sensitivity
0.312 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount EPGN Microplate 96 Wells (12 x 8 Well strips) EPGN Lyophilized Standard 2 100X EPGN Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount EPGN Microplate 96 Wells (12 x 8 Well strips) EPGN Microplate 96 Wells (12 x 8 Well strips) EPGN Lyophilized Standard 2 EPGN Lyophilized Standard 2 100X EPGN Biotinylated Detector Anitbody 1 x 120 uL 100X EPGN Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
EPGN
Protein Name
Epigen
Gene Name URL
EPGN
Nucleotide Accession Number
NM_001270989
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