CTGF Chemi-Luminescent ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cellular communication network factor 2
Gene Aliases
CCN family member 2; connective tissue growth factor; CTGF; HCS24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; insulin-like growth factor-binding protein 8; NOV2.
Gene ID
1490
Accession Number
NP_001892
Reactivity
Homo sapiens|Human
Target
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Type
ELISA Kit
Applications
Chemiluminescent immunoassay
Detection Method
Chemiluminescent
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.37-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 78-101 90 EDTA Plasma (n=5) 93-103 96 Heparin Plasma (n=5) 96-104 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-101 90 Serum (n=5) 78-101 90 EDTA Plasma (n=5) 93-103 96 EDTA Plasma (n=5) 93-103 96 Heparin Plasma (n=5) 96-104 99 Heparin Plasma (n=5) 96-104 99
Sensitivity
109.74 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CTGF Microplate 96 Wells (12 x 8 Well strips) CTGF Lyophilized Standard 2 100X CTGF Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CTGF Microplate 96 Wells (12 x 8 Well strips) CTGF Microplate 96 Wells (12 x 8 Well strips) CTGF Lyophilized Standard 2 CTGF Lyophilized Standard 2 100X CTGF Biotinylated Detector Anitbody 1 x 120 uL 100X CTGF Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
CCN2
Protein Name
Connective tissue growth factor
Gene Name URL
CCN2
Nucleotide Accession Number
NM_001901
Frequently Asked Questions
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