CLCF1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

B-cell stimulatory factor 3; B-cell-stimulating factor 3; BSF3; BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT1; NNT-1; novel neurotrophin-1; NR6.

The microplate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cardiotrophin Like Cytokine Factor 1 (CLCF1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 83-96 93 EDTA Plasma (n=5) 82-101 85 Heparin Plasma (n=5) 90-104 95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 83-96 93 Serum (n=5) 83-96 93 EDTA Plasma (n=5) 82-101 85 EDTA Plasma (n=5) 82-101 85 Heparin Plasma (n=5) 90-104 95 Heparin Plasma (n=5) 90-104 95

Component Amount CLCF1 Microplate 96 Wells (12 x 8 Well strips) CLCF1 Lyophilized Standard 2 100X CLCF1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CLCF1 Microplate 96 Wells (12 x 8 Well strips) CLCF1 Microplate 96 Wells (12 x 8 Well strips) CLCF1 Lyophilized Standard 2 CLCF1 Lyophilized Standard 2 100X CLCF1 Biotinylated Detector Anitbody 1 x 120 uL 100X CLCF1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL