CCL3L1 Chemi-Luminescent ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
C-C motif chemokine ligand 3 like 1|C-C motif chemokine ligand 3 like 3
Gene Aliases
464.2; C-C motif chemokine 3-like 1; chemokine (C-C motif) ligand 3-like 1; chemokine (C-C motif) ligand 3-like 3; chemokine (C-C motif) ligand 3-like, centromeric; D17S1718; G0/G1 switch regulatory protein 19-2; G0S19-2; LD78; LD78BETA; LD78-beta (1-70) ; MIP1AP; PAT 464.2; SCYA3L; SCYA3L1; small inducible cytokine A3-like 1; Small-inducible cytokine A3-like 1; tonsillar lymphocyte LD78 beta protein.
Gene ID
414062|6349
Accession Number
NP_001001437
Reactivity
Homo sapiens|Human
Target
Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta (3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta (3-70) is also a ligand for CCR1, CCR3 and CCR5.
Type
ELISA Kit
Applications
Chemiluminescent immunoassay
Detection Method
Chemiluminescent
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) level in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
6.86-5,000pg/mL
Sensitivity
0.078 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CCL3L1 Microplate 96 Wells (12 x 8 Well strips) CCL3L1 Lyophilized Standard 2 100X CCL3L1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CCL3L1 Microplate 96 Wells (12 x 8 Well strips) CCL3L1 Microplate 96 Wells (12 x 8 Well strips) CCL3L1 Lyophilized Standard 2 CCL3L1 Lyophilized Standard 2 100X CCL3L1 Biotinylated Detector Anitbody 1 x 120 uL 100X CCL3L1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
CCL3L1|CCL3L3
Protein Name
C-C motif chemokine 3-like 1
Gene Name URL
CCL3L1|CCL3L3
Nucleotide Accession Number
NM_001001437
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