SERPING1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

C1 esterase inhibitor; C1IN; C1INH; C1-inhibiting factor; C1-inhibitor; C1NH; complement component 1 inhibitor; epididymis secretory sperm binding protein; HAE1; HAE2; plasma protease C1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1; serpin G1; serpin peptidase inhibitor clade G member 1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1.

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

The microplate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement 1 Inhibitor (C1INH) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 78-97 91 EDTA Plasma (n=5) 94-101 98 Heparin Plasma (n=5) 93-104 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-97 91 Serum (n=5) 78-97 91 EDTA Plasma (n=5) 94-101 98 EDTA Plasma (n=5) 94-101 98 Heparin Plasma (n=5) 93-104 98 Heparin Plasma (n=5) 93-104 98

Component Amount SERPING1 Microplate 96 Wells (12 x 8 Well strips) SERPING1 Lyophilized Standard 2 100X SERPING1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount SERPING1 Microplate 96 Wells (12 x 8 Well strips) SERPING1 Microplate 96 Wells (12 x 8 Well strips) SERPING1 Lyophilized Standard 2 SERPING1 Lyophilized Standard 2 100X SERPING1 Biotinylated Detector Anitbody 1 x 120 uL 100X SERPING1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL