What is the catalog number for BMP4 Chemi-Luminescent ELISA Kit (Pig)?

The catalog number for BMP4 Chemi-Luminescent ELISA Kit (Pig) is OKCD03334-96W.

What sizes are available for BMP4 Chemi-Luminescent ELISA Kit (Pig)?

BMP4 Chemi-Luminescent ELISA Kit (Pig) is available in the following size(s): 96 Well.

Is BMP4 Chemi-Luminescent ELISA Kit (Pig) in stock?

BMP4 Chemi-Luminescent ELISA Kit (Pig) is currently made to order / not held in immediate stock. Contact us for lead time.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

BMP4 Chemi-Luminescent ELISA Kit (Pig)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Bone morphogenetic protein 4

Gene Aliases

Bone morphogenetic protein 4.

Gene ID

100113425

Accession Number

NP_001094501

Reactivity

Porcine|Sus scrofa

Target

Bone morphogenetic protein 4 precursor

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

13.7-10,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 102 EDTA Plasma (n=5) 79-93 89 Heparin Plasma (n=5) 85-104 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 102 Serum (n=5) 98-105 102 EDTA Plasma (n=5) 79-93 89 EDTA Plasma (n=5) 79-93 89 Heparin Plasma (n=5) 85-104 92 Heparin Plasma (n=5) 85-104 92

Sensitivity

0.156 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Bmp4 Microplate 96 Wells (12 x 8 Well strips) Bmp4 Lyophilized Standard 2 100X Bmp4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Bmp4 Microplate 96 Wells (12 x 8 Well strips) Bmp4 Microplate 96 Wells (12 x 8 Well strips) Bmp4 Lyophilized Standard 2 Bmp4 Lyophilized Standard 2 100X Bmp4 Biotinylated Detector Anitbody 1 x 120 uL 100X Bmp4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL