ANXA1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Annexin A1; Annexin I; annexin I (lipocortin I) ; annexin-1; ANX1; calpactin II; calpactin-2; chromobindin-9; epididymis secretory sperm binding protein; Lipocortin I; LPC1; p35; phospholipase A2 inhibitory protein.

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544) . Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity) . Promotes resolution of inflammation and wound healing (PubMed:25664854) . Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854) . Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149) . Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549) . Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549) . Has no effect on unstimulated T cells (PubMed:17008549) . Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149) . Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660) . Has high affinity for Ca (2+) and can bind up to eight Ca (2+) ions (By similarity) . Displays Ca (2+) -dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678) . Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca (2+) -dependent interaction between phagosomes and the actin cytoskeleton (By similarity) .

The microplate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A1 (ANXA1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 85-103 89 EDTA Plasma (n=5) 93-104 101 Heparin Plasma (n=5) 91-101 96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 85-103 89 Serum (n=5) 85-103 89 EDTA Plasma (n=5) 93-104 101 EDTA Plasma (n=5) 93-104 101 Heparin Plasma (n=5) 91-101 96 Heparin Plasma (n=5) 91-101 96

Component Amount ANXA1 Microplate 96 Wells (12 x 8 Well strips) ANXA1 Lyophilized Standard 2 100X ANXA1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount ANXA1 Microplate 96 Wells (12 x 8 Well strips) ANXA1 Microplate 96 Wells (12 x 8 Well strips) ANXA1 Lyophilized Standard 2 ANXA1 Lyophilized Standard 2 100X ANXA1 Biotinylated Detector Anitbody 1 x 120 uL 100X ANXA1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL