What is the catalog number for TPSAB1 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for TPSAB1 Chemi-Luminescent ELISA Kit (Human) is OKCD03283-96W.

What sizes are available for TPSAB1 Chemi-Luminescent ELISA Kit (Human)?

TPSAB1 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TPSAB1 Chemi-Luminescent ELISA Kit (Human) in stock?

TPSAB1 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

TPSAB1 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Alpha fetoprotein

Gene Aliases

AFPD; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; FETA; HPAFP.

Gene ID

174

Accession Number

NP_062195

Reactivity

Homo sapiens|Human

Target

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (AFP) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (AFP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-Fetoprotein (AFP) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

68.6-50,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 94-105 98 EDTA Plasma (n=5) 94-102 99 Heparin Plasma (n=5) 90-99 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94-105 98 Serum (n=5) 94-105 98 EDTA Plasma (n=5) 94-102 99 EDTA Plasma (n=5) 94-102 99 Heparin Plasma (n=5) 90-99 93 Heparin Plasma (n=5) 90-99 93

Sensitivity

0.14 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Lyophilized Standard 2 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Lyophilized Standard 2 TPSAB1 Lyophilized Standard 2 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL