AMBP Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

A1M; bikunin; complex-forming glycoprotein heterogeneous in charge; EDC1; growth-inhibiting protein 19; HCP; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI.

The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin (a1M) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-1-Microglobulin (a1M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-1-Microglobulin (a1M) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 92-105 99 EDTA Plasma (n=5) 92-105 102 Heparin Plasma (n=5) 82-103 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92-105 99 Serum (n=5) 92-105 99 EDTA Plasma (n=5) 92-105 102 EDTA Plasma (n=5) 92-105 102 Heparin Plasma (n=5) 82-103 88 Heparin Plasma (n=5) 82-103 88

Component Amount AMBP Microplate 96 Wells (12 x 8 Well strips) AMBP Lyophilized Standard 2 100X AMBP Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount AMBP Microplate 96 Wells (12 x 8 Well strips) AMBP Microplate 96 Wells (12 x 8 Well strips) AMBP Lyophilized Standard 2 AMBP Lyophilized Standard 2 100X AMBP Biotinylated Detector Anitbody 1 x 120 uL 100X AMBP Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL