PYY3 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Peptide YY 3 (PYY3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Peptide YY 3 (PYY3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Peptide YY 3 (PYY3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Peptide YY 3 (PYY3) and unlabeled Peptide YY 3 (PYY3) (Standards or samples) with the pre-coated antibody specific to Peptide YY 3 (PYY3) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY 3 (PYY3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY 3 (PYY3) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 94-104 97 EDTA Plasma (n=5) 78-95 84 Heparin Plasma (n=5) 86-102 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94-104 97 Serum (n=5) 94-104 97 EDTA Plasma (n=5) 78-95 84 EDTA Plasma (n=5) 78-95 84 Heparin Plasma (n=5) 86-102 98 Heparin Plasma (n=5) 86-102 98

Component Amount Anti-PYY3 Microplate 96 Wells (12 x 8 Well strips) PYY3 Lyophilized Standard 2 x 1 ng 100X PYY3 Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PYY3 Microplate 96 Wells (12 x 8 Well strips) Anti-PYY3 Microplate 96 Wells (12 x 8 Well strips) PYY3 Lyophilized Standard 2 x 1 ng PYY3 Lyophilized Standard 2 x 1 ng 100X PYY3 Biotin Complex 1 x 120 uL 100X PYY3 Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Peptide YY 3 (PYY3) . No significant cross-reactivity or interference between Peptide YY 3 (PYY3) and analogues was observed.