GHRH ELISA Kit (Pig)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone Releasing Hormone (GHRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone Releasing Hormone (GHRH) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 89-103 93 EDTA Plasma (n=5) 93-105 99 Heparin Plasma (n=5) 85-102 95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 89-103 93 Serum (n=5) 89-103 93 EDTA Plasma (n=5) 93-105 99 EDTA Plasma (n=5) 93-105 99 Heparin Plasma (n=5) 85-102 95 Heparin Plasma (n=5) 85-102 95

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This assay has high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH) . No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.