EGR1 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

A530045N19Rik; early growth response protein 1; egr; Egr-; Egr-1; ETR10; ETR103; Krox; Krox-; Krox-1; Krox24; Krox-24; Krox-24 nuclear protein; nerve growth factor-induced protein A; NG; NGF; NGF1; NGF1-A; NGFIA; NGFI-A; TI; TIS8; transcription factor Zif268; Zen; Zenk; Zfp-; Zfp-6; Zif26; Zif268; zinc finger protein Krox-24.

Transcriptional regulator (PubMed:8336701, PubMed:8703054, PubMed:15958557) . Recognizes and binds to the DNA sequence 5'-GCG (T/G) GGGCG-3' (EGR-site) in the promoter region of target genes (PubMed:8703054, PubMed:15958557, PubMed:2028256, PubMed:8939742) . Binds double-stranded target DNA, irrespective of the cytosine methylation status (By similarity) . Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia (PubMed:11100120, PubMed:15958557) . Plays a role in the regulation of cell survival, proliferation and cell death (PubMed:15265859, PubMed:15958557) . Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation (PubMed:15958557) . Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy (PubMed:15265859) . Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia (PubMed:11100120) . Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (PubMed:8703054) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Growth Response Protein 1 (EGR1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Early Growth Response Protein 1 (EGR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Growth Response Protein 1 (EGR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Early Growth Response Protein 1 (EGR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount Anti-EGR1 Microplate 96 Wells (12 x 8 Well strips) EGR1 Lyophilized Standard 2 100X Biotinylated EGR1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-EGR1 Microplate 96 Wells (12 x 8 Well strips) Anti-EGR1 Microplate 96 Wells (12 x 8 Well strips) EGR1 Lyophilized Standard 2 EGR1 Lyophilized Standard 2 100X Biotinylated EGR1 Detector Antibody 1 x 120 uL 100X Biotinylated EGR1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Early Growth Response Protein 1 (EGR1) . No significant cross-reactivity or interference between Early Growth Response Protein 1 (EGR1) and analogues was observed.