EGFL7 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

EGF-like protein 7; epidermal growth factor-like protein 7; multiple EGF-like domains protein 7; multiple epidermal growth factor-like domains protein 7; NEU1 protein; NOTCH4-like protein; vascular endothelial statin; VE-s; VE-statin; Zneu; Zneu1.

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF) -BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 87-101 92 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 90-104 97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 87-101 92 Serum (n=5) 87-101 92 EDTA Plasma (n=5) 98-105 102 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 90-104 97 Heparin Plasma (n=5) 90-104 97

Component Amount Anti-EGFL7 Microplate 96 Wells (12 x 8 Well strips) EGFL7 Lyophilized Standard 2 100X Biotinylated EGFL7 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-EGFL7 Microplate 96 Wells (12 x 8 Well strips) Anti-EGFL7 Microplate 96 Wells (12 x 8 Well strips) EGFL7 Lyophilized Standard 2 EGFL7 Lyophilized Standard 2 100X Biotinylated EGFL7 Detector Antibody 1 x 120 uL 100X Biotinylated EGFL7 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of EGF Like Domain Protein, Multiple 7 (EGFL7) . No significant cross-reactivity or interference between EGF Like Domain Protein, Multiple 7 (EGFL7) and analogues was observed.