CYR61 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

3CH61; AI325051; CCN; CCN family member 1; Cyr6; Cyr61; cysteine rich protein 61; cysteine-rich angiogenic inducer 61; IBP-10; IGF-binding protein 10; Igfbp10; IGFBP-10; insulin-like growth factor-binding protein 10; intermediate early; protein CYR61.

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity) . CYR61-mediated gene regulation is dependent on heparin-binding (By similarity) . Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity) . Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 92-101 95 EDTA Plasma (n=5) 81-104 88 Heparin Plasma (n=5) 81-90 86 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92-101 95 Serum (n=5) 92-101 95 EDTA Plasma (n=5) 81-104 88 EDTA Plasma (n=5) 81-104 88 Heparin Plasma (n=5) 81-90 86 Heparin Plasma (n=5) 81-90 86

Component Amount Anti-CYR61 Microplate 96 Wells (12 x 8 Well strips) CYR61 Lyophilized Standard 2 100X Biotinylated CYR61 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CYR61 Microplate 96 Wells (12 x 8 Well strips) Anti-CYR61 Microplate 96 Wells (12 x 8 Well strips) CYR61 Lyophilized Standard 2 CYR61 Lyophilized Standard 2 100X Biotinylated CYR61 Detector Antibody 1 x 120 uL 100X Biotinylated CYR61 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) . No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.