CCR2 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

C-C chemokine receptor type 2; C-C CHEMOKINE RECEPTOR TYPE 2 (C-C CKR-2) (CC-CKR-2) (CCR-2) (CCR2) (JE/FIC RECEPTOR) (MCP-1 RECEPTOR) ; C-C CKR-2; CC-CKR-; Cc-ckr-2; CCR; CCR-2; Ccr2a; Ccr2b; chemoattractant protein-1 receptor; chemokine (C-C) receptor 2; CKR; Ckr2; Ckr2a; Ckr2b; Cmkbr; Cmkbr2; JE/FIC receptor; MCP-1 receptor; MIP-1 alphaR; mJe-r.

Receptor for the CCL2, CCL7 and CCL12 chemokines (PubMed:8996246) . Receptor for the beta-defensin DEFB106A/DEFB106B (By similarity) . Transduces a signal by increasing intracellular calcium ion levels (PubMed:8996246) . Upon CCL2 ligation, mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 2 (CCR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-C-Motif Receptor 2 (CCR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 78-90 82 EDTA Plasma (n=5) 86-94 90 Heparin Plasma (n=5) 81-101 86 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-90 82 Serum (n=5) 78-90 82 EDTA Plasma (n=5) 86-94 90 EDTA Plasma (n=5) 86-94 90 Heparin Plasma (n=5) 81-101 86 Heparin Plasma (n=5) 81-101 86

Component Amount Anti-CCR2 Microplate 96 Wells (12 x 8 Well strips) CCR2 Lyophilized Standard 2 100X Biotinylated CCR2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CCR2 Microplate 96 Wells (12 x 8 Well strips) Anti-CCR2 Microplate 96 Wells (12 x 8 Well strips) CCR2 Lyophilized Standard 2 CCR2 Lyophilized Standard 2 100X Biotinylated CCR2 Detector Antibody 1 x 120 uL 100X Biotinylated CCR2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 2 (CCR2) . No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 2 (CCR2) and analogues was observed.