What is the catalog number for CAR3 ELISA Kit (Human)?

The catalog number for CAR3 ELISA Kit (Human) is OKCD02411-96W.

What sizes are available for CAR3 ELISA Kit (Human)?

CAR3 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CAR3 ELISA Kit (Human) in stock?

CAR3 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

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Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

CAR3 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Carbonic Anhydrase III, Muscle Specific (CA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Carbonic Anhydrase III, Muscle Specific (CA3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Carbonic anhydrase 3

Gene Aliases

CAIII; CA-III; Car3; carbonate dehydratase III; carbonic anhydrase 3; Carbonic anhydrase III; carbonic anhydrase III, muscle specific; carbonic anhydrase IIII; epididymis secretory sperm binding protein Li 167mP; HEL-S-167mP.

Gene ID

761

Accession Number

NP_031632.2

Reactivity

Homo sapiens|Human

Target

Reversible hydration of carbon dioxide.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase III, Muscle Specific (CA3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase III, Muscle Specific (CA3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase III, Muscle Specific (CA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase III, Muscle Specific (CA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids

Detection Range

7.8-500pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 95 EDTA Plasma (n=5) 79-92 87 Heparin Plasma (n=5) 97-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 95 Serum (n=5) 91-99 95 EDTA Plasma (n=5) 79-92 87 EDTA Plasma (n=5) 79-92 87 Heparin Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 97-105 101

Sensitivity

3.7 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Anti-CAR3 Microplate 96 Wells (12 x 8 Well strips) CAR3 Lyophilized Standard 2 100X Biotinylated CAR3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CAR3 Microplate 96 Wells (12 x 8 Well strips) Anti-CAR3 Microplate 96 Wells (12 x 8 Well strips) CAR3 Lyophilized Standard 2 CAR3 Lyophilized Standard 2 100X Biotinylated CAR3 Detector Antibody 1 x 120 uL 100X Biotinylated CAR3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase III, Muscle Specific (CA3) . No significant cross-reactivity or interference between Carbonic Anhydrase III, Muscle Specific (CA3) and analogues was observed.