Nicotinic Acid Adenine Dinucleotide Phosphate ELISA Kit

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

NAADP; NAADP+; 1-[ (2R,3R,4S,5R) -5-[[[[ (2R,3R,4R,5R) -5- (6-aminopurin-9-yl) -3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-3,4-dihydroxyoxolan-2-yl]pyridin-1-ium-3-carboxylic acid; Nicotinic acid adenine dinuc

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and unlabeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) (Standards or samples) with the pre-coated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 80-101 88 EDTA plasma (n=5) 83-98 87 heparin plasma (n=5) 79-103 83 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 80-101 88 serum (n=5) 80-101 88 EDTA plasma (n=5) 83-98 87 EDTA plasma (n=5) 83-98 87 heparin plasma (n=5) 79-103 83 heparin plasma (n=5) 79-103 83

Component Amount Anti-Nicotinic Acid Adenine Dinucleotide Phosphate Microplate 96 Wells (12 x 8 Well Strips) Nicotinic Acid Adenine Dinucleotide Phosphate Lyophilized Standard 2 100X Nicotinic Acid Adenine Dinucleotide Phosphate Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Nicotinic Acid Adenine Dinucleotide Phosphate Microplate 96 Wells (12 x 8 Well Strips) Anti-Nicotinic Acid Adenine Dinucleotide Phosphate Microplate 96 Wells (12 x 8 Well Strips) Nicotinic Acid Adenine Dinucleotide Phosphate Lyophilized Standard 2 Nicotinic Acid Adenine Dinucleotide Phosphate Lyophilized Standard 2 100X Nicotinic Acid Adenine Dinucleotide Phosphate Biotin Complex 120 uL 100X Nicotinic Acid Adenine Dinucleotide Phosphate Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL