What is the catalog number for 1-Desamino 8D Arginine Vasopressin ELISA Kit?

The catalog number for 1-Desamino 8D Arginine Vasopressin ELISA Kit is OKCD02265-96W.

What sizes are available for 1-Desamino 8D Arginine Vasopressin ELISA Kit?

1-Desamino 8D Arginine Vasopressin ELISA Kit is available in the following size(s): 96 Well.

Is 1-Desamino 8D Arginine Vasopressin ELISA Kit in stock?

1-Desamino 8D Arginine Vasopressin ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

1-Desamino 8D Arginine Vasopressin ELISA Kit

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Aliases

DESMOPRESSIN; 16679-58-6; DDAVP; UNII-ENR1LLB0FP

Reactivity

All Species

Target

Desmopressin is a Factor VIII Activator and Vasopressin Analog. The physiologic effect of desmopressin is by means of Increased Coagulation Factor VIII Activity and Increased Coagulation Factor VIII Concentration. The chemical classification of desmopressin is Vasopressins.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Detection Method

Colorimetric

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids.

Detection Range

246.9-20,000pg/mL

Recovery

Matrix Recovery Range (%) Average (%) serum (n=5) 84-105 102 EDTA plasma (n=5) 90-101 93 heparin plasma (n=5) 78-96 83 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 84-105 102 serum (n=5) 84-105 102 EDTA plasma (n=5) 90-101 93 EDTA plasma (n=5) 90-101 93 heparin plasma (n=5) 78-96 83 heparin plasma (n=5) 78-96 83

Sensitivity

39.5 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Anti-1-Desamino 8D Arginine Vasopressin Microplate 96 Wells (12 x 8 Well Strips) 1-Desamino 8D Arginine Vasopressin Lyophilized Standard 2 100X 1-Desamino 8D Arginine Vasopressin Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-1-Desamino 8D Arginine Vasopressin Microplate 96 Wells (12 x 8 Well Strips) Anti-1-Desamino 8D Arginine Vasopressin Microplate 96 Wells (12 x 8 Well Strips) 1-Desamino 8D Arginine Vasopressin Lyophilized Standard 2 1-Desamino 8D Arginine Vasopressin Lyophilized Standard 2 100X 1-Desamino 8D Arginine Vasopressin Biotin Complex 120 uL 100X 1-Desamino 8D Arginine Vasopressin Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL