Acetylcarnitine ELISA Kit

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

L-Acetylcarnitine (ALCAR or ALC) is an acetic acid ester of carnitine that facilitates movement of acetyl-CoA into the matrices of mammalian mitochondria during the oxidation of fatty acids. In addition to his metabolic role, acetyl-L-carnitine posses unique neuroprotective, neuromodulatory, and neurotrophic properties this may play an important role in counteracting various disease processes.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcarnitine (ALCAR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 80-95 82 EDTA plasma (n=5) 90-98 94 heparin plasma (n=5) 80-102 99 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 80-95 82 serum (n=5) 80-95 82 EDTA plasma (n=5) 90-98 94 EDTA plasma (n=5) 90-98 94 heparin plasma (n=5) 80-102 99 heparin plasma (n=5) 80-102 99

Component Amount Anti-Acetylcarnitine Microplate 96 Wells (12 x 8 Well Strips) Acetylcarnitine Lyophilized Standard 2 100X Acetylcarnitine Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Acetylcarnitine Microplate 96 Wells (12 x 8 Well Strips) Anti-Acetylcarnitine Microplate 96 Wells (12 x 8 Well Strips) Acetylcarnitine Lyophilized Standard 2 Acetylcarnitine Lyophilized Standard 2 100X Acetylcarnitine Biotin Complex 120 uL 100X Acetylcarnitine Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL