IGF2BP3 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Cancer/testis antigen 98; CT98; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; IMP3; IMP-3; insulin-like growth factor 2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH domain-containing protein overexpressed in cancer; KOC; KOC1; VICKZ family member 3; VICKZ3.

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs) . This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Serum (n=5) 81-98 92 EDTA Plasma (n=5) 88-102 92 Heparin Plasma (n=5) 88-97 92 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 81-98 92 Serum (n=5) 81-98 92 EDTA Plasma (n=5) 88-102 92 EDTA Plasma (n=5) 88-102 92 Heparin Plasma (n=5) 88-97 92 Heparin Plasma (n=5) 88-97 92

Component Amount Anti-IGF2BP3 Microplate 96 Wells (12 x 8 Well Strips) IGF2BP3 Lyophilized Standard 2 x 10 ng 100X Biotinylated IGF2BP3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-IGF2BP3 Microplate 96 Wells (12 x 8 Well Strips) Anti-IGF2BP3 Microplate 96 Wells (12 x 8 Well Strips) IGF2BP3 Lyophilized Standard 2 x 10 ng IGF2BP3 Lyophilized Standard 2 x 10 ng 100X Biotinylated IGF2BP3 Detector Antibody 120 uL 100X Biotinylated IGF2BP3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) . No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.