CRIM1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CRIM-1; cysteine rich transmembrane BMP regulator 1 (chordin-like) ; cysteine-rich motor neuron 1 protein; cysteine-rich repeat-containing protein S52; S52.

May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Serum (n=5) 90-99 95 EDTA Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 95-104 99 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 90-99 95 Serum (n=5) 90-99 95 EDTA Plasma (n=5) 99-105 102 EDTA Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 95-104 99 Heparin Plasma (n=5) 95-104 99

Component Amount Anti-CRIM1 Microplate 96 Wells (12 x 8 Well Strips) CRIM1 Lyophilized Standard 2 x 20 ng 100X Biotinylated CRIM1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CRIM1 Microplate 96 Wells (12 x 8 Well Strips) Anti-CRIM1 Microplate 96 Wells (12 x 8 Well Strips) CRIM1 Lyophilized Standard 2 x 20 ng CRIM1 Lyophilized Standard 2 x 20 ng 100X Biotinylated CRIM1 Detector Antibody 120 uL 100X Biotinylated CRIM1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) . No significant cross-reactivity or interference between Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) and analogues was observed.