CEACAM8 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen related cell adhesion molecule 8; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b; CD67; CD67 antigen; CGM6; NCA-95; non-specific cross-reacting antigen NCA-95.

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190) . Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190) . Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Serum (n=5) 86-99 95 EDTA Plasma (n=5) 85-105 96 Heparin Plasma (n=5) 97-104 101 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 86-99 95 Serum (n=5) 86-99 95 EDTA Plasma (n=5) 85-105 96 EDTA Plasma (n=5) 85-105 96 Heparin Plasma (n=5) 97-104 101 Heparin Plasma (n=5) 97-104 101

Component Amount Anti-CEACAM8 Microplate 96 Wells (12 x 8 Well Strips) CEACAM8 Lyophilized Standard 2 x 1 ng 100X Biotinylated CEACAM8 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CEACAM8 Microplate 96 Wells (12 x 8 Well Strips) Anti-CEACAM8 Microplate 96 Wells (12 x 8 Well Strips) CEACAM8 Lyophilized Standard 2 x 1 ng CEACAM8 Lyophilized Standard 2 x 1 ng 100X Biotinylated CEACAM8 Detector Antibody 120 uL 100X Biotinylated CEACAM8 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) . No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) and analogues was observed.