CEACAM3 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Carcinoembryonic antigen CGM1; carcinoembryonic antigen gene family member 1; carcinoembryonic antigen related cell adhesion molecule 3; carcinoembryonic antigen-related cell adhesion molecule 3; CD66D; CD66d antigen; CEA; CGM1; nonspecific cross-reacting antigen; W264; W282.

Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Serum (n=5) 89-103 96 EDTA Plasma (n=5) 79-94 90 Heparin Plasma (n=5) 91-101 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 89-103 96 Serum (n=5) 89-103 96 EDTA Plasma (n=5) 79-94 90 EDTA Plasma (n=5) 79-94 90 Heparin Plasma (n=5) 91-101 96 Heparin Plasma (n=5) 91-101 96

Component Amount Anti-CEACAM3 Microplate 96 Wells (12 x 8 Well Strips) CEACAM3 Lyophilized Standard 2 x 1 ng 100X Biotinylated CEACAM3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CEACAM3 Microplate 96 Wells (12 x 8 Well Strips) Anti-CEACAM3 Microplate 96 Wells (12 x 8 Well Strips) CEACAM3 Lyophilized Standard 2 x 1 ng CEACAM3 Lyophilized Standard 2 x 1 ng 100X Biotinylated CEACAM3 Detector Antibody 120 uL 100X Biotinylated CEACAM3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) . No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) and analogues was observed.