SERPINA10 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Serpin A10 (SERPINA10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Serpin A10 (SERPINA10) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Protein Z-dependent protease inhibitor; Protein-Z dependent proteinase inhibitor; PZ-dependent protease inhibitor; PZI; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10; serpin A10; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10; ZPI.

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin A10 (SERPINA10) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serpin A10 (SERPINA10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serpin A10 (SERPINA10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serpin A10 (SERPINA10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Sodium Citrate Plasma (n=5) 84-105 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Sodium Citrate Plasma (n=5) 84-105 88 Sodium Citrate Plasma (n=5) 84-105 88

Component Amount Anti-SERPINA10 Microplate 96 Wells (12 x 8 Well Strips) SERPINA10 Lyophilized Standard 2 x 80 ng 100X Biotinylated SERPINA10 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SERPINA10 Microplate 96 Wells (12 x 8 Well Strips) Anti-SERPINA10 Microplate 96 Wells (12 x 8 Well Strips) SERPINA10 Lyophilized Standard 2 x 80 ng SERPINA10 Lyophilized Standard 2 x 80 ng 100X Biotinylated SERPINA10 Detector Antibody 120 uL 100X Biotinylated SERPINA10 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Matrices listed below were spiked with certain level of recombinant Protein Z Dependent Protease Inhibitor (ZPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Protein Z Dependent Protease Inhibitor (ZPI) in samples.