Ccl21c ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

C-C motif chemokine 21c|chemokine (C-C motif) ligand 21B (leucine) |chemokine (C-C motif) ligand 21C (leucine) |chemokine (C-C motif) ligand 21D|predicted gene 10591|predicted gene 13304

6CKBAC;6CKBAC1;6Cki;6Ckine; AI159701; beta-chemokine exodus-2; C-C motif chemokine 21b; C-C motif chemokine 21c; c-C motif chemokine 21c-like; Ccl21b; Ccl21c; Ccl21-leu; chemokine (C-C motif) ligand 21C (leucine) ; CKb9; Exodus; exodus-2; Gm21541; S; Scya; Scya2; Scya21; Scya21a; Scya21b; Scya21c; secondary lymphoid organ chemokine; SLC; small inducible cytokine A21; small inducible cytokine A21c; small-inducible cytokine A21a; small-inducible cytokine A21b; Small-inducible cytokine A21c; TCA; TCA4; thymus-derived chemotactic agent 4.

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secondary Lymphoid Tissue Chemokine (SLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secondary Lymphoid Tissue Chemokine (SLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) Serum (n=5) 81-98 95 EDTA Plasma (n=5) 85-92 88 Heparin Plasma (n=5) 87-96 92 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 81-98 95 Serum (n=5) 81-98 95 EDTA Plasma (n=5) 85-92 88 EDTA Plasma (n=5) 85-92 88 Heparin Plasma (n=5) 87-96 92 Heparin Plasma (n=5) 87-96 92

Component Amount Anti-Ccl21c Microplate 96 Wells (12 x 8 Well Strips) Ccl21c Lyophilized Standard 2 x 500 pg 100X Biotinylated Ccl21c Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ccl21c Microplate 96 Wells (12 x 8 Well Strips) Anti-Ccl21c Microplate 96 Wells (12 x 8 Well Strips) Ccl21c Lyophilized Standard 2 x 500 pg Ccl21c Lyophilized Standard 2 x 500 pg 100X Biotinylated Ccl21c Detector Antibody 120 uL 100X Biotinylated Ccl21c Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Secondary Lymphoid Tissue Chemokine (SLC) . No significant cross-reactivity or interference between Secondary Lymphoid Tissue Chemokine (SLC) and analogues was observed.