LN High Sensitivity ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Laminin (LN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CLM; LAMA; LAMB2; laminin A chain; laminin B1 chain; laminin B2 chain; laminin subunit alpha-1; laminin subunit beta-1; laminin subunit gamma-1; laminin, alpha 1; laminin, beta 1; laminin, gamma 1 (formerly LAMB2) ; Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; laminin-10 subunit gamma; laminin-11 subunit gamma; Laminin-12 subunit beta; Laminin-2 subunit beta; laminin-2 subunit gamma; laminin-3 subunit alpha; laminin-3 subunit gamma; laminin-4 subunit gamma; Laminin-6 subunit beta; laminin-6 subunit gamma; laminin-7 subunit gamma; Laminin-8 subunit beta; laminin-8 subunit gamma; laminin-9 subunit gamma; LIS5; PTBHS; S-LAM alpha; S-LAM gamma; S-LAM-alpha; S-laminin subunit alpha; S-laminin subunit gamma.

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.|Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Laminin (LN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Laminin (LN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Laminin (LN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Laminin (LN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 92-101 96 EDTA Plasma (n=5) 84-105 88 Heparin Plasma (n=5) 98-105 102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92-101 96 Serum (n=5) 92-101 96 EDTA Plasma (n=5) 84-105 88 EDTA Plasma (n=5) 84-105 88 Heparin Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 98-105 102

Component Amount Anti-LN Microplate 96 Wells (12 x 8 Well Strips) LN Lyophilized Standard 2 100X Biotinylated LN Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-LN Microplate 96 Wells (12 x 8 Well Strips) Anti-LN Microplate 96 Wells (12 x 8 Well Strips) LN Lyophilized Standard 2 LN Lyophilized Standard 2 100X Biotinylated LN Detector Antibody 120 uL 100X Biotinylated LN Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Laminin (LN) . No significant cross-reactivity or interference between Laminin (LN) and analogues was observed.