What sizes are available for Glutamic Acid Decarboxylase Antibody ELISA Kit (Human)?

Glutamic Acid Decarboxylase Antibody ELISA Kit (Human) is available in the following size(s): 96 Well.

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Glutamic Acid Decarboxylase Antibody ELISA Kit (Human)

Q: What is the catalog number for Glutamic Acid Decarboxylase Antibody ELISA Kit (Human)?

The catalog number for Glutamic Acid Decarboxylase Antibody ELISA Kit (Human) is OKCD01298-96W.

Q: Is Glutamic Acid Decarboxylase Antibody ELISA Kit (Human) in stock?

Glutamic Acid Decarboxylase Antibody ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Glutamate decarboxylase 1

Gene Aliases

67 kDa glutamic acid decarboxylase; CPSQ1; DEE89; GAD; GAD-67; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa) ; Glutamate decarboxylase 67 kDa isoform; SCP.

Gene ID

2571

Reactivity

Homo sapiens|Human

Target

Catalyzes the production of GABA.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Detection Method

Colorimetric

Assay Principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100ng/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 95 EDTA Plasma (n=5) 79-93 85 Heparin Plasma (n=5) 81-99 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 95 Serum (n=5) 91-99 95 EDTA Plasma (n=5) 79-93 85 EDTA Plasma (n=5) 79-93 85 Heparin Plasma (n=5) 81-99 88 Heparin Plasma (n=5) 81-99 88

Sensitivity

0.59 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Glutamic Acid Decarboxylase antibody Microplate 96 Wells (12 x 8 Well strips) Glutamic Acid Decarboxylase antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Glutamic Acid Decarboxylase antibody Microplate 96 Wells (12 x 8 Well strips) Glutamic Acid Decarboxylase antibody Microplate 96 Wells (12 x 8 Well strips) Glutamic Acid Decarboxylase antibody Lyophilized Standard 2 x XXX Glutamic Acid Decarboxylase antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL