Mannose Binding Lectin Antibody ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

COLEC1; collectin-1; HSMBPC; mannan-binding lectin; Mannan-binding protein; Mannose-binding lectin; mannose-binding lectin (protein C) 2, soluble (opsonic defect) ; mannose-binding lectin 2, soluble (opsonic defect) ; mannose-binding protein C; MBL; MBL2D; MBP; MBP1; MBP-C; MBPD.

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 EDTA Plasma (n=5) 79-104 99 Heparin Plasma (n=5) 81-105 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 Serum (n=5) 95-104 101 EDTA Plasma (n=5) 79-104 99 EDTA Plasma (n=5) 79-104 99 Heparin Plasma (n=5) 81-105 92 Heparin Plasma (n=5) 81-105 92

Component Amount Mannose Binding Lectin antibody Microplate 96 Wells (12 x 8 Well strips) Mannose Binding Lectin antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Mannose Binding Lectin antibody Microplate 96 Wells (12 x 8 Well strips) Mannose Binding Lectin antibody Microplate 96 Wells (12 x 8 Well strips) Mannose Binding Lectin antibody Lyophilized Standard 2 x XXX Mannose Binding Lectin antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL