TIGIT ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSIG9, VSTM3; VSTM3; Washington University cell adhesion molecule; WUCAM.

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 78-91 86 EDTA plasma (n=5) 93-104 99 heparin plasma (n=5) 92-101 97 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 78-91 86 serum (n=5) 78-91 86 EDTA plasma (n=5) 93-104 99 EDTA plasma (n=5) 93-104 99 heparin plasma (n=5) 92-101 97 heparin plasma (n=5) 92-101 97

Component Amount Anti-TIGIT Microplate 96 Wells (12 x 8 Well Strips) TIGIT Lyophilized Standard 2 x 20 ng 100X Biotinylated TIGIT Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-TIGIT Microplate 96 Wells (12 x 8 Well Strips) Anti-TIGIT Microplate 96 Wells (12 x 8 Well Strips) TIGIT Lyophilized Standard 2 x 20 ng TIGIT Lyophilized Standard 2 x 20 ng 100X Biotinylated TIGIT Detector Antibody 120 uL 100X Biotinylated TIGIT Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) . No significant cross-reactivity or interference between T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) and analogues was observed.