CPSF1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Cleavage and polyadenylation specific factor 1, 160kDa; cleavage and polyadenylation specificity factor 160 kDa subunit; cleavage and polyadenylation specificity factor subunit 1; CPSF 160 kDa subunit; CPSF160; HSU37012; MYP27; P/cl.18; polyadenylation specificity factor.

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly (A) polymerase and other factors to bring about cleavage and poly (A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cleavage And Polyadenylation Specific Factor 1 (CPSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cleavage And Polyadenylation Specific Factor 1 (CPSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount Anti-CPSF1 Microplate 96 Wells (12 x 8 Well Strips) CPSF1 Lyophilized Standard 2 x 20 ng 100X Biotinylated CPSF1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CPSF1 Microplate 96 Wells (12 x 8 Well Strips) Anti-CPSF1 Microplate 96 Wells (12 x 8 Well Strips) CPSF1 Lyophilized Standard 2 x 20 ng CPSF1 Lyophilized Standard 2 x 20 ng 100X Biotinylated CPSF1 Detector Antibody 120 uL 100X Biotinylated CPSF1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Cleavage And Polyadenylation Specific Factor 1 (CPSF1) . No significant cross-reactivity or interference between Cleavage And Polyadenylation Specific Factor 1 (CPSF1) and analogues was observed.