KRAS ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; CFC2; c-Ki-ras; c-Ki-ras2; c-Kirsten-ras protein; C-K-RAS; 'C-K-RAS; GTPase KRas; KI-RAS; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; K-Ras; K-Ras 2; K-ras p21 protein; KRAS1; KRAS2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; NS; NS3; OES; oncogene KRAS2; PR310 c-K-ras oncogene; RALD; RASK2; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog.

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621) . Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838) . Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 89-97 92 EDTA plasma (n=5) 78-97 87 heparin plasma (n=5) 99-105 102 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-97 92 serum (n=5) 89-97 92 EDTA plasma (n=5) 78-97 87 EDTA plasma (n=5) 78-97 87 heparin plasma (n=5) 99-105 102 heparin plasma (n=5) 99-105 102

Component Amount Anti-KRAS Microplate 96 Wells (12 x 8 Well Strips) KRAS Lyophilized Standard 2 x 20 ng 100X Biotinylated KRAS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-KRAS Microplate 96 Wells (12 x 8 Well Strips) Anti-KRAS Microplate 96 Wells (12 x 8 Well Strips) KRAS Lyophilized Standard 2 x 20 ng KRAS Lyophilized Standard 2 x 20 ng 100X Biotinylated KRAS Detector Antibody 120 uL 100X Biotinylated KRAS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) . No significant cross-reactivity or interference between V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) and analogues was observed.