LILRA3 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CD85 antigen-like family member E; CD85E; HM31; HM43; ILT6; ILT-6; immunoglobulin-like transcript 6; leucocyte Ig-like receptor A3; leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor subfamily A member 3; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 3; LIR4; LIR-4; monocyte inhibitory receptor HM43/HM31.

Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 85-102 98 EDTA plasma (n=5) 82-96 87 heparin plasma (n=5) 79-101 86 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 85-102 98 serum (n=5) 85-102 98 EDTA plasma (n=5) 82-96 87 EDTA plasma (n=5) 82-96 87 heparin plasma (n=5) 79-101 86 heparin plasma (n=5) 79-101 86

Component Amount Anti-LILRA3 Microplate 96 Wells (12 x 8 Well Strips) LILRA3 Lyophilized Standard 2 x 20 ng 100X Biotinylated LILRA3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-LILRA3 Microplate 96 Wells (12 x 8 Well Strips) Anti-LILRA3 Microplate 96 Wells (12 x 8 Well Strips) LILRA3 Lyophilized Standard 2 x 20 ng LILRA3 Lyophilized Standard 2 x 20 ng 100X Biotinylated LILRA3 Detector Antibody 120 uL 100X Biotinylated LILRA3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) . No significant cross-reactivity or interference between Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) and analogues was observed.