CD9 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

5H9 antigen; antigen CD9; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 antigen (p24) ; cell growth-inhibiting gene 2 protein; DRAP-27; leukocyte antigen MIC3; MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; tetraspanin-29; TSPAN29; TSPAN-29.

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Motility Related Protein (MRP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Motility Related Protein (MRP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Motility Related Protein (MRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Motility Related Protein (MRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 83-90 86 EDTA plasma (n=5) 96-104 101 heparin plasma (n=5) 97-105 101 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 83-90 86 serum (n=5) 83-90 86 EDTA plasma (n=5) 96-104 101 EDTA plasma (n=5) 96-104 101 heparin plasma (n=5) 97-105 101 heparin plasma (n=5) 97-105 101

Component Amount Anti-CD9 Microplate 96 Wells (12 x 8 Well Strips) CD9 Lyophilized Standard 2 x 2 ng 100X Biotinylated CD9 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CD9 Microplate 96 Wells (12 x 8 Well Strips) Anti-CD9 Microplate 96 Wells (12 x 8 Well Strips) CD9 Lyophilized Standard 2 x 2 ng CD9 Lyophilized Standard 2 x 2 ng 100X Biotinylated CD9 Detector Antibody 120 uL 100X Biotinylated CD9 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1) . No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.