CD300LF ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CD300 antigen like family member F; CD300 antigen-like family member F; CLIM1; CLM1; CLM-1; CMRF35-like molecule 1; CMRF-35-like molecule 1; DC-derived Ig-like receptor 2; DIg; Digr2; F730004D16Rik; IgSF; IgSF13; IRE; IREM1; leukocyte mono-Ig-like receptor 3; LM; LMIR3; MAIR-5; MAIR-V; myeloid-associated immunoglobulin-like receptor 5; Pi; Pigr3.

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:17438331) . Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells (PubMed:21865548) . Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (PubMed:26768664) . Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand (PubMed:23123064) . May act as a coreceptor for interleukin 4 (IL-4) . Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (PubMed:26124135) . Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (By similarity) . Inhibits osteoclast formation (PubMed:14662855) . Induces macrophage cell death upon engagement (PubMed:18097021) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immune Receptor Expressed On Myeloid Cells 1 (IREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Immune Receptor Expressed On Myeloid Cells 1 (IREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount Anti-Cd300lf Microplate 96 Wells (12 x 8 Well Strips) Cd300lf Lyophilized Standard 2 x 100 ng 100X Biotinylated Cd300lf Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Cd300lf Microplate 96 Wells (12 x 8 Well Strips) Anti-Cd300lf Microplate 96 Wells (12 x 8 Well Strips) Cd300lf Lyophilized Standard 2 x 100 ng Cd300lf Lyophilized Standard 2 x 100 ng 100X Biotinylated Cd300lf Detector Antibody 120 uL 100X Biotinylated Cd300lf Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Immune Receptor Expressed On Myeloid Cells 1 (IREM1) . No significant cross-reactivity or interference between Immune Receptor Expressed On Myeloid Cells 1 (IREM1) and analogues was observed.