NBL1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

C1orf151-NBL1; C1orf151-NBL1 read-through protein; D1S1733E; DAN; DAN domain family member 1; DAND1; differential screening-selected gene aberrant in neuroblastoma; MICOS10-NBL1 read-through protein; MINOS1-NBL1; MINOS1-NBL1 readthrough; NB; NBL1; neuroblastoma 1, DAN family BMP antagonist; neuroblastoma candidate region, suppression of tumorigenicity 1; neuroblastoma suppressor of tumorigenicity 1; NO3; Protein N03; Zinc finger protein DAN.

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount Anti-NBL1 Microplate 96 Wells (12 x 8 Well Strips) NBL1 Lyophilized Standard 2 x 10 ng 100X Biotinylated NBL1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-NBL1 Microplate 96 Wells (12 x 8 Well Strips) Anti-NBL1 Microplate 96 Wells (12 x 8 Well Strips) NBL1 Lyophilized Standard 2 x 10 ng NBL1 Lyophilized Standard 2 x 10 ng 100X Biotinylated NBL1 Detector Antibody 120 uL 100X Biotinylated NBL1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) . No significant cross-reactivity or interference between Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) and analogues was observed.