SLURP1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Secreted Ly6/uPAR Related Protein 1 (SLURP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Secreted Ly6/uPAR Related Protein 1 (SLURP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Anti-neoplastic urinary protein; ANUP; ARS; ARS component B; ARS (component B) -81/S; ArsB; LY6LS; LY6-MT; lymphocyte antigen 6-like secreted; MDM; secreted Ly6/uPAR related protein 1; secreted Ly-6/uPAR-related protein 1; SLURP-1.

Has an antitumor activity (PubMed:8742060) . Was found to be a marker of late differentiation of the skin. Implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin (PubMed:14721776, PubMed:17008884) . In vitro down-regulates keratinocyte proliferation; the function may involve the proposed role as modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-7-dependent nAChR currents in an allosteric manner (PubMed:14506129, PubMed:26905431) . In T cells may be involved in regulation of intracellular Ca (2+) signaling (PubMed:17286989) . Seems to have a immunomodulatory function in the cornea (By similarity) . The function may implicate a possible role as a scavenger receptor for PLAU thereby blocking PLAU-dependent functions of PLAUR such as in cell migration and proliferation (PubMed:25168896) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Ly6/uPAR Related Protein 1 (SLURP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Ly6/uPAR Related Protein 1 (SLURP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Ly6/uPAR Related Protein 1 (SLURP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secreted Ly6/uPAR Related Protein 1 (SLURP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 85-101 95 EDTA plasma (n=5) 92-101 96 heparin plasma (n=5) 79-102 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 85-101 95 serum (n=5) 85-101 95 EDTA plasma (n=5) 92-101 96 EDTA plasma (n=5) 92-101 96 heparin plasma (n=5) 79-102 96 heparin plasma (n=5) 79-102 96

Component Amount Anti-SLURP1 Microplate 96 Wells (12 x 8 Well Strips) SLURP1 Lyophilized Standard 2 x 10 ng 100X Biotinylated SLURP1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SLURP1 Microplate 96 Wells (12 x 8 Well Strips) Anti-SLURP1 Microplate 96 Wells (12 x 8 Well Strips) SLURP1 Lyophilized Standard 2 x 10 ng SLURP1 Lyophilized Standard 2 x 10 ng 100X Biotinylated SLURP1 Detector Antibody 120 uL 100X Biotinylated SLURP1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Secreted Ly6/uPAR Related Protein 1 (SLURP1) . No significant cross-reactivity or interference between Secreted Ly6/uPAR Related Protein 1 (SLURP1) and analogues was observed.