BPIFA1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

BA49G10.5; BPI fold-containing family A member 1; ligand-binding protein RYA3; lung-specific protein X; LUNX; NASG; nasopharyngeal carcinoma-related protein; palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; PLUNC; protein Plunc; secretory protein in upper respiratory tracts; short PLUNC1; SPLUNC1; SPURT; tracheal epithelium enriched protein; Tracheal epithelium-enriched protein; von Ebner protein Hl.

Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS) . Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Palate/Lung And Nasal Epithelium Associated Protein (PLUNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 98-105 101 _x005F EDTA plasma (n=5) 94-101 98 _x005F heparin plasma (n=5) 94-101 97 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 98-105 101 serum (n=5) 98-105 101 EDTA plasma (n=5) 94-101 98 EDTA plasma (n=5) 94-101 98 heparin plasma (n=5) 94-101 97 heparin plasma (n=5) 94-101 97

Component Amount _x005F Anti-BPIFA1 Microplate 96 Wells (12 x 8 Well Strips) _x005F BPIFA1 Lyophilized Standard 2 x 10 ng _x005F 100X Biotinylated BPIFA1 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-BPIFA1 Microplate 96 Wells (12 x 8 Well Strips) Anti-BPIFA1 Microplate 96 Wells (12 x 8 Well Strips) BPIFA1 Lyophilized Standard 2 x 10 ng BPIFA1 Lyophilized Standard 2 x 10 ng 100X Biotinylated BPIFA1 Detector Antibody 120 uL 100X Biotinylated BPIFA1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) ._x005F No significant cross-reactivity or interference between Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) and analogues was observed.