CADM1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

BL2; cell adhesion molecule 1; IGSF4; IGSF4A; immunoglobulin superfamily member 4; immunoglobulin superfamily, member 4D variant 1; immunoglobulin superfamily, member 4D variant 2; Necl-2; NECL2; nectin-like 2; nectin-like protein 2; RA175; sgIGSF; spermatogenic immunoglobulin superfamily; ST17; sTSLC-1; synaptic cell adhesion molecule; SYNCAM; synCAM1; truncated CADM1 protein; TSLC1; TSLC-1; TSLC1/Nectin-like 2/IGSF4; tumor suppressor in lung cancer 1.

Mediates homophilic cell-cell adhesion in a Ca (2+) -independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca (2+) -independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity) . May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule 1 (CADM1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Adhesion Molecule 1 (CADM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule 1 (CADM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cell Adhesion Molecule 1 (CADM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 85-104 93 EDTA plasma (n=5) 85-97 92 heparin plasma (n=5) 85-99 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 85-104 93 serum (n=5) 85-104 93 EDTA plasma (n=5) 85-97 92 EDTA plasma (n=5) 85-97 92 heparin plasma (n=5) 85-99 88 heparin plasma (n=5) 85-99 88

Component Amount Anti-CADM1 Microplate 96 Wells (12 x 8 Well Strips) CADM1 Lyophilized Standard 2 x 10 ng 100X Biotinylated CADM1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CADM1 Microplate 96 Wells (12 x 8 Well Strips) Anti-CADM1 Microplate 96 Wells (12 x 8 Well Strips) CADM1 Lyophilized Standard 2 x 10 ng CADM1 Lyophilized Standard 2 x 10 ng 100X Biotinylated CADM1 Detector Antibody 120 uL 100X Biotinylated CADM1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Cell Adhesion Molecule 1 (CADM1) . No significant cross-reactivity or interference between Cell Adhesion Molecule 1 (CADM1) and analogues was observed.