What is the catalog number for NSMAF ELISA Kit (Human)?

The catalog number for NSMAF ELISA Kit (Human) is OKCD00413-96W.

What sizes are available for NSMAF ELISA Kit (Human)?

NSMAF ELISA Kit (Human) is available in the following size(s): 96 Well.

Is NSMAF ELISA Kit (Human) in stock?

NSMAF ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

NSMAF ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Neutral Sphingomyelinase Activation Associated Factor (NSMAF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Neutral Sphingomyelinase Activation Associated Factor (NSMAF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Neutral sphingomyelinase activation associated factor

Gene Aliases

Factor associated with neutral sphingomyelinase activation; factor associated with N-SMase activation; FAN; GRAMD5; neutral sphingomyelinase (N-SMase) activation associated factor; protein FAN.

Gene ID

8439

Accession Number

NP_001138244

Reactivity

Homo sapiens|Human

Target

Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE) . Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutral Sphingomyelinase Activation Associated Factor (NSMAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neutral Sphingomyelinase Activation Associated Factor (NSMAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Detection Range

0.156-10ng/mL

Recovery

Matrix Recovery Range (%) Average (%) 99-105 102 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) 99-105 102 99-105 102

Sensitivity

< 0.054 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Anti-NSMAF Microplate 96 Wells (12 x 8 Well Strips) NSMAF Lyophilized Standard 2 x 10 ng 100X Biotinylated NSMAF Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-NSMAF Microplate 96 Wells (12 x 8 Well Strips) Anti-NSMAF Microplate 96 Wells (12 x 8 Well Strips) NSMAF Lyophilized Standard 2 x 10 ng NSMAF Lyophilized Standard 2 x 10 ng 100X Biotinylated NSMAF Detector Antibody 120 uL 100X Biotinylated NSMAF Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Notes

Formerly GWB-KBBUG6

Specificity

This assay has high sensitivity and excellent specificity for detection of Neutral Sphingomyelinase Activation Associated Factor (NSMAF) . No significant cross-reactivity or interference between Neutral Sphingomyelinase Activation Associated Factor (NSMAF) and analogues was observed.