NAXE ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

AIBP; AI-BP; APOA1BP; apoA-I binding protein; apolipoprotein A-I-binding protein; NAD (P) H-hydrate epimerase; NAD (P) HX epimerase; PEBEL; yjeF N-terminal domain-containing protein 1; yjeF_N1; YJEFN1.

Catalyzes the epimerization of the S- and R-forms of NAD (P) HX, a damaged form of NAD (P) H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD (P) H-hydrate dehydratase to allow the repair of both epimers of NAD (P) HX.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 Binding Protein (APOA1BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein A1 Binding Protein (APOA1BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 83-97 91 EDTA plasma (n=5) 92-105 96 heparin plasma (n=5) 86-94 90 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 83-97 91 serum (n=5) 83-97 91 EDTA plasma (n=5) 92-105 96 EDTA plasma (n=5) 92-105 96 heparin plasma (n=5) 86-94 90 heparin plasma (n=5) 86-94 90

Component Amount Anti-NAXE Microplate 96 Wells (12 x 8 Well Strips) NAXE Lyophilized Standard 2 x 10 ng 100X Biotinylated NAXE Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-NAXE Microplate 96 Wells (12 x 8 Well Strips) Anti-NAXE Microplate 96 Wells (12 x 8 Well Strips) NAXE Lyophilized Standard 2 x 10 ng NAXE Lyophilized Standard 2 x 10 ng 100X Biotinylated NAXE Detector Antibody 120 uL 100X Biotinylated NAXE Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 Binding Protein (APOA1BP) . No significant cross-reactivity or interference between Apolipoprotein A1 Binding Protein (APOA1BP) and analogues was observed.