MYH7 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Cardiac muscle myosin heavy chain 7 beta; CMD1S; CMH1; MPD1; MYHCB; myHC-beta; myhc-slow; myopathy, distal 1; myosin 7; Myosin heavy chain 7; myosin heavy chain beta-subunit; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM.

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 81-96 85 EDTA plasma (n=5) 95-103 99 heparin plasma (n=5) 79-104 91 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 81-96 85 serum (n=5) 81-96 85 EDTA plasma (n=5) 95-103 99 EDTA plasma (n=5) 95-103 99 heparin plasma (n=5) 79-104 91 heparin plasma (n=5) 79-104 91

Component Amount Anti-MYH7 Microplate 96 Wells (12 x 8 Well Strips) MYH7 Lyophilized Standard 2 x 10 ng 100X Biotinylated MYH7 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-MYH7 Microplate 96 Wells (12 x 8 Well Strips) Anti-MYH7 Microplate 96 Wells (12 x 8 Well Strips) MYH7 Lyophilized Standard 2 x 10 ng MYH7 Lyophilized Standard 2 x 10 ng 100X Biotinylated MYH7 Detector Antibody 120 uL 100X Biotinylated MYH7 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) . No significant cross-reactivity or interference between Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) and analogues was observed.