ROS1 ELISA Kit (Rat)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

C-ros oncogene 1 , receptor tyrosine kinase; c-Ros receptor tyrosine kinase; c-ros-1; heart - derived c - ros - 1 proto - oncogene; proto-oncogene c-Ros; proto-oncogene c-Ros-1; proto-oncogene tyrosine-protein kinase ROS; proto-oncogene tyrosine-protein kinase ROS-like; receptor tyrosine kinase c-ros oncogene 1; Ros1 proto-oncogene; ROS1C; v-ros UR2 sarcoma virus oncogene homolog 1.

Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2 (By similarity) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 97-104 101 EDTA plasma (n=5) 80-94 84 heparin plasma (n=5) 84-96 89 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 97-104 101 serum (n=5) 97-104 101 EDTA plasma (n=5) 80-94 84 EDTA plasma (n=5) 80-94 84 heparin plasma (n=5) 84-96 89 heparin plasma (n=5) 84-96 89

Component Amount Anti-Ros1 Microplate 96 Wells (12 x 8 Well Strips) Ros1 Lyophilized Standard 2 x 1 ng 100X Biotinylated Ros1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ros1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Ros1 Microplate 96 Wells (12 x 8 Well Strips) Ros1 Lyophilized Standard 2 x 1 ng Ros1 Lyophilized Standard 2 x 1 ng 100X Biotinylated Ros1 Detector Antibody 120 uL 100X Biotinylated Ros1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) . No significant cross-reactivity or interference between C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) and analogues was observed.