SLPI ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:9126337) . Modulates the innate immune response after bacterial infection (PubMed:12615907) . Contributes to regulate the inflammatory and immune responses to the intracellular parasite L.major (PubMed:25030421) . Down-regulates responses to bacterial lipopolysaccharide (LPS) (PubMed:9039268, PubMed:12615907, PubMed:25030421) . Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:11017147, PubMed:12615907) . Has antimicrobial activity against mycobacteria, but not against salmonella (PubMed:18322212) . Contributes to normal resistance against infection by M.tuberculosis (PubMed:18322212) . Required for normal resistance to L.major (PubMed:25030421) . Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (PubMed:11017147, PubMed:25030421) . Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (By similarity) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretory Leukocyte Peptidase Inhibitor (SLPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secretory Leukocyte Peptidase Inhibitor (SLPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 78-89 83 _x005F EDTA plasma (n=5) 94-101 97 _x005F heparin plasma (n=5) 91-99 95 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 78-89 83 serum (n=5) 78-89 83 EDTA plasma (n=5) 94-101 97 EDTA plasma (n=5) 94-101 97 heparin plasma (n=5) 91-99 95 heparin plasma (n=5) 91-99 95

Component Amount _x005F Anti-Slpi Microplate 96 Wells (12 x 8 Well Strips) _x005F Slpi Lyophilized Standard 2 x 1 ng _x005F 100X Biotinylated Slpi Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-Slpi Microplate 96 Wells (12 x 8 Well Strips) Anti-Slpi Microplate 96 Wells (12 x 8 Well Strips) Slpi Lyophilized Standard 2 x 1 ng Slpi Lyophilized Standard 2 x 1 ng 100X Biotinylated Slpi Detector Antibody 120 uL 100X Biotinylated Slpi Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Secretory Leukocyte Peptidase Inhibitor (SLPI) ._x005F No significant cross-reactivity or interference between Secretory Leukocyte Peptidase Inhibitor (SLPI) and analogues was observed.