CXCL17 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

6-Cys CXCL17; chemokine (C-X-C motif) ligand 17; C-X-C motif chemokine 17; Dcip1; dendritic cell and monocyte chemokine-like protein; DMC; UNQ473; VCC1; VCC-1; VEGF coregulated chemokine 1; VEGF co-regulated chemokine 1.

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:16455961, PubMed:23115081) . Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774, PubMed:23115081) . Acts as an anti-inflammatory in the stomach (PubMed:23115081) . May play a role in the innate defense against infections (PubMed:17307946) . Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions (PubMed:25411203) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF Co Regulated Chemokine 1 (VCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGF Co Regulated Chemokine 1 (VCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 87-104 96 EDTA plasma (n=5) 80-94 87 heparin plasma (n=5) 85-97 89 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 87-104 96 serum (n=5) 87-104 96 EDTA plasma (n=5) 80-94 87 EDTA plasma (n=5) 80-94 87 heparin plasma (n=5) 85-97 89 heparin plasma (n=5) 85-97 89

Component Amount Anti-CXCL17 Microplate 96 Wells (12 x 8 Well Strips) CXCL17 Lyophilized Standard 2 x 1 ng 100X Biotinylated CXCL17 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CXCL17 Microplate 96 Wells (12 x 8 Well Strips) Anti-CXCL17 Microplate 96 Wells (12 x 8 Well Strips) CXCL17 Lyophilized Standard 2 x 1 ng CXCL17 Lyophilized Standard 2 x 1 ng 100X Biotinylated CXCL17 Detector Antibody 120 uL 100X Biotinylated CXCL17 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of VEGF Co Regulated Chemokine 1 (VCC1) . No significant cross-reactivity or interference between VEGF Co Regulated Chemokine 1 (VCC1) and analogues was observed.