What is the catalog number for ACTA2 ELISA Kit (Rat)?

The catalog number for ACTA2 ELISA Kit (Rat) is OKCA02613-96W.

What sizes are available for ACTA2 ELISA Kit (Rat)?

ACTA2 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is ACTA2 ELISA Kit (Rat) in stock?

ACTA2 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

ACTA2 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46

Gene ID

100009271

Accession Number

NP_001095152

Reactivity

Rat

Target

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology ACTA2 ELISA Kit (Rat) (OKCA02613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACTA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard ACTA2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma And Tissue Homogenates

Detection Range

0.78 ng/mL -50 ng/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 EDTA Plasma (n=4) 93 89-98 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA Plasma (n=4) 93 89-98 EDTA Plasma (n=4) 93 89-98