What is the catalog number for SDC2 ELISA Kit (Rat)?

The catalog number for SDC2 ELISA Kit (Rat) is OKCA02597-96W.

What sizes are available for SDC2 ELISA Kit (Rat)?

SDC2 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is SDC2 ELISA Kit (Rat) in stock?

SDC2 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

SDC2 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

HSPG

Gene ID

25615

Accession Number

NP_037214

Reactivity

Rat

Target

Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Sdc2 ELISA Kit (Rat) (OKCA02597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sdc2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Sdc2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

3.12 - 200 ng/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 85-96 EDTA Plasma (n=4) 96 90-101 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 85-96 Serum (n=5) 91 85-96 EDTA Plasma (n=4) 96 90-101 EDTA Plasma (n=4) 96 90-101